Biology Reference
In-Depth Information
Generally, one helical turn of amylose is composed of
approximately six repeating glucose units when linear molecules
of small cross-sectional area, e.g., fatty acids, are included [27]. The
repeat distance of the helix of amylose was reported as ca. 0.80 nm
[27], whereas the length of one unit of PTHF was calculated as ca.
0.60 nm (see Fig. 7.6). Therefore, 4.5 repeating glucose units in
amylose correspond to the length of one PTHF unit (Fig. 7.6). On the
basis of these calculations, the ratio of the proton numbers between
amylose and PTHF in the ideal inclusion complex can be calculated.
The integrated ratio of the signal due to amylose to the signal due to
PTHF in the
1
H NMR spectrum of the product was in good agreement
with the calculated value [25]. This also supported the structure of
the inclusion complex.
The molecular weights of the amylose and PTHF in the inclusion
complex were evaluated by means of
1
H NMR measurements when
Amylose
HO
HO
OH
O
O
O
O
O
O
HO
HO
HO
O
O
O
OH
OH
OH
HO
HO
HO
OH
OH
OH
HO
HO
HO
O
O
O
O
O
O
OH
OH
OH
O
O
O
HO
HO
HO
OH
OH
OH
O
O
O
HO
HO
HO
HO
HO
HO
HO
HO
HO
O
O
O
O
O
O
OH
OH
OH
OH
OH
OH
OH
OH
OH
O
O
O
HO
HO
HO
OH
OH
OH
O
O
O
HO
HO
HO
O
O
O
0.80 nm
0.60 nm
6.0 glucose units
1.0 unit in PTHF
0.60 nm
PTHF
4.5 glucose units
O
O
O
Figure 7.6
Illustration of repeat distance of amylose helix and unit length
of PTHF.
