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OH
O
OH
OH
inorganic
phosphate
O
+
O
HO
OH
HO
HO
OH
cellobiose
cellobiose
phosphorylase
OH
O
OH
O
HO
O
+
OH
OH
HO
O
HO
O
P
O
HO
OH
glucose
Glc-1-P
Figure 3.5
Catalysis of cellobiose phosphorylase.
OH
O
OH
O
O
HO
OH
R
HO
O
OH
+
X
O
P
O
HO
OH
HO
HO
OH
O
Glc-1-P
X = O, S
cellodextrin phosphorylase
OH
O
OH
R
O
OH
inorganic phosphate
X
+
O
HO
OH
O
HO
OH
H
O
OH
HO
n
cellooligosaccharides
Figure 3.6
Cellodextrin
phosphorylase-catalyzed
synthesis
of
cellooligosaccharides.
-
d
-xylose
1-phosphate (Xyl-1-P) as a glycosyl donor and xylose-containing
disaccharides as a glycosyl acceptor [19]. Therefore, the enzymatic
synthesis of a library of
Cellodextrin phosphorylase was found to recognize
α
4)-hetero-glucose and xylose-based
oligosaccharides was attempted by the cellodextrin phosphorylase-
catalyzed glycosylation using Glc-1-P or Xyl-1-P as a donor and
cellobiose, xylobiose, Glc-
β
-(1
→
4)-Glc as an
acceptor (Fig. 3.7). Consequently, the enzymatic glycosylation
by the cellodextrin phosphorylase catalysis produced all six
β
-(1
→
4)-Xyl, or Xyl-
β
-(1
→
