Phosphorylase-Catalyzed Enzymatic Glycosylation - Engineering of Polysaccharide Materials - page 22

Table 3.1 Characteristics of phosphorylases

EC Enzyme Mechanism Substrate Product

2.4.1.1 (

α

-Glucan) phosphorylase Retention Glc-

α

-1,4-Glc

α

-Glc-1-P

2.4.1.7 Sucrose phosphorylase Retention Glc-

α

-1,2-Fru

α

-Glc-1-P

2.4.1.8 Maltose phosphorylase Inversion Glc-

α

-1,4-Glc

β

-Glsc-1-P

2.4.1.20 Cellobiose phosphorylase Inversion Glc-

β

-1,4-Glc

β

-Glc-1-P

2.4.1.30

β

-1,3-Oligoglucan phosphorylase Inversion Glc-

β

-1,3-Glc

α

-Glc-1-P

2.4.1.31 Laminaribiose phosphorylase Inversion Glc-

β

-1,3-Glc

α

-Glc-1-P

2.4.1.49 Cellodextrin phosphorylase Inversion Glc-

β

-1,4-Glc

β

-Glc-1-P

2.4.1.64 Trehalose phosphorylase Inversion Glc-

α

1,

α

1-Glc

β

-Glc-1-P

2.4.1.97 Laminarin phosphorylase Inversion Glc-

β

-1,3-Glc

α

-Glc-1-P

2.4.1.211

β

-1,3-Galactosyl- N -acetylhexosamine phosphorylase Inversion Gal-

β

-1,3-GlyNAc

α

-Gal-1-P

2.4.1.230 Kojibiose phosphorylase Inversion Glc-

α

-1,2-Glc

β

-Glc-1-P

2.4.1.231 Trehalose phosphorylase Retention Glc-

α

1,

α

1-Glc

α

-Glc-1-P

— Chitobiose phosphorylase Inversion GlcNAc-

β

-1,4-GlcNAc

α

-GlcNAc-1-P