Biology Reference
In-Depth Information
as
[11], have been
isolated. The BE genes were expressed in various host strains, and
the enzymes were well characterized.
Branched glucan can be produced by the combined action of
phosphorylase and BE on Glc-1-P in the presence of an adequate
primer (Fig. 9.2) [12,13]. The molecular weight and branching
pattern of the product are expected to be controlled by the Glc-1-P/
primer ratio as in the case of amylose synthesis by the phosphorylase-
catalyzed polymerization, and by the relative BE/phosphorylase
activity ratio, respectively. Thus, various branched glucans were
produced by using different BE/phosphorylase activity ratios. The
branched glucans produced at high BE/phosphorylase activity ratios
had more frequently branching points than those produced at low
BE/phosphorylase ratios.
B. stearothermphilus
[10] and
Aquifex aeolicus
Figure 9.2
Synthesis of branched glucan by combined use of phosph-
orylase and BE.
