Chemistry Reference
In-Depth Information
Denmark,sweetenersincludingACS-KandASPareseparatedbyHPLConaC-18column(5mm,
250mm×4.6mm)equippedwithaguardcolumn,elutedisocraticallywithamixtureofmethanol
andwaterbufferedwithpotassiumhydrogenphosphate,andmeasuredspectrophotometricallyat
220nm(Lethetal.2007).FortheestimationoftheintakeofACS-KandASPfromsoftdrinks
foragroupofPortugueseteenagestudents,reversed-phaseLCwasusedwithaHichromC
18
col-
umn(5μm,250mm×4.6mm)andabufferedmobilephase[KH
2
PO
4
0.02M/acetonitrile(90:10,
v/v)/phosphoricacid]at1mL/min.ThepHwasrigorouslycontrolledat4.2-4.3foranadequate
resolutionbetweenASPandbenzoicacid,whichispresentinmostanalyzedsamples.Detection
wasperformedwithaUVdetectorat220nm.Anexternalstandardmethodisusedforquantiica-
tion(Linoetal.2008).AnisocraticseparationofsomefoodadditiveswithHPLCanalysiswas
carriedoutonaShimadzuclassLC-VPHPLCsystemwithanautosampler(SIL-10ADVP)anda
diode-arraydetector(SPD-M10AVP).A5-μmYMC-ODSPackAMcolumn(250mm×4.6mm
I.D.)wasusedfortheanalysis.Inthisstudy,threedifferentvaluesofthemobilephaseacetonitrile
content(15%,20%,and25%,v/v)wereprepared.ThepHvalueswereadjustedto4.0withglacial
aceticacid.Thelowratewas0.8mL/min,andthevolumeinjectedwas20μL.ForACS-K,detec-
tionfollowedat230nm,andforASP,detectionfollowedat203nm.ThisHPLC-UVprocedure
wasabletoseparateACS-KandASPfromotheradditivesincoladrinksandininstantpowder
drinks.Therecoveryachievedis99%-101%,theLODis0.2to3.1μg/g,andtheRSDis1.0%to
2.2%forallanalytesdetermined(Demiralayetal.2006).
AnHPLC-UVprocedurewasabletoseparateASPandACS-Kfromsaccharin,benzoicacid,
sorbicacid,Ponceau4R,SunsetYellow,andTartrazineinsoftdrinksusingaLiChrosorbC
18
col-
umn (10μm, 250 mm × 4.6 mm) and a mobile phase that consisted of MeOH-phosphate buffer
(pH4).Therecoveryachievedis98.6%to102.3%withalimitofdetectionof0.1to3mg/lforall
analytesdetermined(Dossietal.2006).
AnHPLC-evaporativelightscanningdetector(HPLC-ELSD)procedurewasabletoseparate
ASPandACS-Kfromsaccharin,cyclamate,sucralose,dulcin,alitame,NTM,andNHDCinnon-
carbonatedsoftdrinks,cannedorbottledfruits,andyogurtsusingC
18
stationaryphaseandamobile
phase that consisted of TEA formate buffer-MeOH-acetonitrile (ACN). The limit of detection
is15 μg/g,andtheRSDis0.9%to4.5%forallanalytesdetermined(Wasiketal.2007).Anion
pairedHPLC-UVprocedureisabletoseparateACS-Kfromsaccharin,dulcin,preservatives,anti-
oxidants in sugared fruits, soy sauces, and dried roast beef. A Shoko stainless-steel 5C
18
(5μm,
250 mm × 4.6 mm) was used as the stationary phase and the mobile phase consisted of ACN-
aqueousa-hydroxyisobutyricacidsolutioncontaininghexadecyltrimethylammoniumbromide.The
recoveryis81.9%to103.27%,thelimitofdetectionachievedis0.15to3μg/g,andtheRSDis0.3%
to5.69%forallanalytesdetermined(ChenandFu1995).Ahigh-performanceIC-UV-electrolytic
conductivitydetector(HPIC-UV-ELCD)procedureisabletoseparateACS-KandASPfromsac-
charin,cyclamate,andcitricacidindrinksandpowderedtabletopsweeteners.ADionexIonPac
AS4A-SC(254mm×4mm)wasusedasthestationaryphaseandthemobilephaseconsistedof
Na
2
CO
3
.Therecoveryis93%to107%,thelimitofdetectionis0.019to0.044mg/L,andtheRSD
is0.84%to1.38%forallanalytesdetermined(Chenetal.1997).AnHPIC-UVprocedureisable
toseparateACS-KandASPfromsaccharin,benzoicacid,sorbicacid,caffeine,theobromineand
theophylineindrinks,juices,fermentedmilkdrinks,preservedfruits,tabletdrinks,andpowdered
tabletop sweeteners. A Shim-pack IC-A3 (5μm, 150 mm × 4.6 mm) was used as the stationary
phaseandthemobilephaseconsistedofNaH
2
PO
4
(pH8.20)-ACN.Therecoveryis85%to104%,
thelimitofdetectionis4to30mg/L,andtheRSDis1%to5%(ChenandWang2001).Finally,
anHPICsuppressedconductivitydetectorprocedureisabletodetermineACS-KandASPinthe
presenceofsaccharinandcyclamateincarbonatedcoladrinks,fruitjuicedrinks,andpreserved
fruits.ADionexIonpacAS11(250mm×2mm)wasusedasthestationaryphaseandthemobile
phaseconsistedofKOH.Therecoveryis97.96%to105.42%,andthelimitofdetectionis0.019to
0.89 mg/Lforalltheanalytesdetermined(Zhuetal.2005)(Table4.4).
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