Biomedical Engineering Reference
In-Depth Information
Figure 1.2
The restriction enzyme ECOR1, illustrated here, is one of hundreds isolated
from bacteria and used by biotechnologists to cut DNA molecules so that they can be
joined with other DNA molecules. This allows scientists to insert a desired genetic
sequence into a strand of DNA.
the RE based on the sequence of the DNA at each end of the gene.
Many REs make staggered cuts that leave a single-stranded tail of
DNA at each end of the cut piece, which can bind, through the rules
of base pairing, to DNA fragments of other sequences cut by the
same RE.
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