Biomedical Engineering Reference
In-Depth Information
Fig. 8.8.
In vivo morphological and cellular imaging in a developmental biology
animal model. Photograph and cross-sectional OCT images of a
Xenopus laevis
(African frog) tadpole specimen showing developing tissue morphology, as well as
individual cells and corresponding histology
Fig. 8.9.
Tracking cell migration. A population of macrophage cells is tracked in
three dimensions over the course of 3 h using OCT. Macrophage cell migration was
induced with a chemoattractant at one end of the 3D scaffold, separated by a semi-
permeable membrane. The time-dependent positions of the cells are color-coded
white (time = 0 h) and grey (time = 3 h). Modified figure used with permission
from [61]
tissue scaffolds [62, 63]. OCT and MPM provide complementary image data.
OCT can image deep through transparent and highly scattering structures
to reveal the 3D structural information. OCT, however, cannot detect the
presence of a fluorescing particle. In a complementary manner, MPM can lo-
calize fluorescent probes in three-dimensional space and provide insight into
cell function. MPM can detect the fluorescence, but not the microstructure
