Biomedical Engineering Reference
In-Depth Information
140
120
y = 141332x-188397
R
2
= 0.99458
100
80
60
40
20
0
1.3330
1.3332
1.3334
1.3336
1.3338
Refractive Index
Fig. 6.10.
Shift of resonance is linearly related to change in refractive index. The
slope of the plotted curve is used to determine the limit of detection for refractive
index changes.
0.12
Binding
Regeneration
BL
0.10
0.08
Intensity
change
DI-H
2
O
0.06
pH
0.04
0.02
Break due to
data acq.
DI-H
2
O
0.00
0
2000
4000 6000
Time (s)
8000
10000
Fig. 6.11.
The detected intensity in a real time binding experiment is plotted. The
plot illustrates both binding and regeneration phases (BL: biotinylated lectin solu-
tion, DI-H
2
O: deionized water, pH: pH-7 buffer). Binding e
cacy is only measured
between the signal levels when DI-H
2
O is flowed across the sensor.
