Environmental Engineering Reference
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Chlorobium phaeovibrioides 2631
Chlorobium phaeovibrioides DSM 269 T
AL-GSB1
clone PENDANT-30
Chlorobium luteolum DSM 273 T
Chlorobium phaeobacteroides UdG6047
Chlorobium phaeobacteroides UdG6051
Chlorobium phaeobacteroides UdG6046
Chlorobium limicola DSM 245 T
Sapropel S1 (partial 16S rRNA-gene)
Chlorobium clathratiforme PG
Chlorobium clathratiforme DSM 5477
Chlorobium phaeobacteroides DSM 266 T
Chlorobaculum parvum DSM 263 T
Chlorobaculum tepidum ATCC 49652 T
Chlorobium limicola UdG6002
Clathrochloris sulfurica 1
Prosthecochloris aestuarii DSM 271 T
Chloroherpeton thalassium
clone PENDANT-20
AL-GSB2
clone PENDANT-21
Organic Lake surface sediment (partial 16S rRNA-gene)
Cytophaga sp. str.JTB150
0.02
Figure 7. Phylogenetic tree showing the relationship of 16S rDNA sequences of green sulfur
bacteria retrieved from particulate organic matter (POM) of the water column and Holocene
sediment layers of Ace Lake (bold text) to reference sequences obtained from the GenBank
and RDP-II databases. The GSB sequences from Ace Lake were determined from the DGGE
represented in Fig. 6. DGGE-bands with identical melting positions within the gel appeared to
contain identical sequences. As a result, phylotype AL-GSB 1 was recovered from the extant
water column and Holocene sediment layers of Unit I and II, whereas phylotype AL-GSB 2 was
unique to Unit III.
of AL-GSB 1 in the top layers of sediment (Fig. 5f) was comparable to this
ratio observed in the deepest analyzed water layer (Fig. 3d). This ratio was
2 to 3 orders of magnitude higher in the deeper Unit II sediments and varied
significantly between alternating layers (Fig. 5f). The percentage of DNA of
AL-GSB 1 was only around 0.1% within the top 39 cm of sediment (Unit I)
and reached up to 2.5% of the total rDNA pool in sediment layers of Unit II
(Fig. 5g). The amount of DNA of AL-GSB 2 comprised only 0.02% of the total
DNA pool (Fig. 5g).
4. DISCUSSION
4.1 Fate of Ancient DNA and Carotenoids of
Chlorobiaceae
All known species of the Chlorobiaceae are obligate anoxygenic photolitho-
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