Biology Reference
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Figure 3.
High throughput application of cryopreserved mouse sperm.
(a). Genetically modified (GM) male
mice were grouped based on their predominant background (first column), and the number of unique GM lines
within each of the backgrounds is shown (second column). In vitro fertilization was preformed employing either
our new sperm cryopreservation and recovery methods (Frozen) or freshly collected sperm (Fresh). The number
of females and oocytes utilized are shown in the fourth and fifth columns. Differences between the Frozen and
Fresh sperm treatments were assessed using ANOVA and preplanned contrasts. Asterisks within a bar indicate
a significant difference between the treatments within a background. (b). A subset of the previously created
embryos using cryopreserved sperm was transferred directly to pseudopregnant recipients. Those embryos created
using freshly collected sperm were cryopreserved prior to transfer, as shown in column four. Differences between
the two treatments were assessed using ANOVA and preplanned contrasts. Asterisks within a bar indicate a
significant difference between the treatments within a background. No p-value is reported in those instances
where the sample size was too small for statistical analysis.
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