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Genetic engineering
of a Bacterial Artiicial
chromosome clone of
Modified Vaccinia Virus
Ankara (MVA)
Matthew G. Cottingham, Rikke F. Andersen,
Alexandra J. Spencer, Saroj Saurya, Julie Furze,
Adrian V. S. Hill and Sarah C. Gilbert
AbstrAct
The production, manipulation and rescue of a bacterial artificial chromo-
some clone of Vaccinia virus (VAC-BAC) in order to expedite construc-
tion of expression vectors and mutagenesis of the genome has been described
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