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modifications, and drafted the manuscript. YH developed the transfer of λ Red-
driven mutations to P. ananatis strains differed from SC17(0). LIG constructed all
recombinant plasmids, expressing λ Red genes, tested their function initially in E.
coli, and edited the manuscript. TMK performed the λ Red-driven modifications
of the chromosome of P. ananatis SC17(0) strain. IGA performed λ Red-driven
oligos integration into chromosome of P. ananatis SC17 strain. SVM supervised
and coordinated the work and edited the manuscript. All authors have read and
approved the final version of the manuscript.
Acknowledgements
Authors acknowledge Prof. B.L. Wanner kindly provided us with BW25113/
pKD46 strain. This study was carried out at the request of and with financial sup-
port from the Ajinomoto Co.
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