Human Neural Stem Cells Genetically Modified to Overexpress Akt1 Provide Neuroprotection and Functional Improvement in Mouse Stroke Model - Genetic Engineering: Recent Developments in Applications - page 299

Functional recovery in icH Animals by hnsc
transplantation
Motor performance of ICH animals receiving PBS, F3 Killed, F3 or F3 Akt1 hN-
SCs was determined by the rotarod and neurology score (Figures 3A, B). The ICH
mice receiving F3.Akt1 hNSCs showed improved behavioral performance on the
rotarod compared with the PBS, F3.killed control groups, and the effect persisted
for at least up to 8 weeks post-transplantation (PT, the point at which animals
were sacrificed) (Figure 3A). Significant difference in rotarod test performance in
F3.Akt1 vs F3 groups detected during the period of only 5 weeks PT (P<0.05).
The F3.Akt1 transplantation group also showed marked improvement in the limb
placement beginning 2 weeks PT and persisting for at least up to 8 weeks (Figure
3B). No significant difference in behavioral performance in F3.Akt1 vs F3 groups
detected during the period of 1~8weeks PT.
Figure 3. Behavioral improvement demonstrated in intracerebral hemorrhage (ICH) mice transplanted with F3
or F3.Akt1 human NSCs. A: Rotarod test. F3.Akt1-transplanted group showed better performance than PBS
controls or F3 cell group, killed F3.Akt1 cell group 8 days onward, and these benefits continued up to 8 weeks
post-transplantation (* P<0.05). B: In the modified limb placement test, F3.Akt1-transplanted group showed
better performance than PBS, F3 or killed F3.Akt1 group (* P<0.05).