Biology Reference
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Dox was administered to the mice through Dox-containing food, which was
custom made by Bio-Serv (Frenchtown, NJ) to contain 2 g Dox per kilogram of
food. The nutrition content of the Dox food (e.g. 19% protein and 8.6% fat)
was very similar to the regular diet (20% protein, 9% fat) used in our colony.
Therefore the switching of food types did not result in change of cholesterol levels
in mice.
Acknowledgements
We thank Gary Felsenfeld for providing pJC13-1, Hermann Bujard for
pUHG10-3 and pUHD15-1neo, Wolfgang Hillen for pCMV-TetR(B/E)-KRAB,
Douglas Powell for statistical analysis, and Vincent Keng for comments on the
manuscript. We thank Susan Reid and Janet Blair-Flynn for excellent technical
assistance. Requests for the TIGRE ES clones should be addressed to GNP.
Author contributions
Conceived and designed the experiments: HZ KH LM LT KB GP GAG. Per-
formed the experiments: HZ KH LM MP GG AR BS YL EO FK PR OS ID ES
AG. Analyzed the data: HZ KH KB AG GAG. Contributed reagents/materials/
analysis tools: FK ES LT GP. Wrote the paper: HZ KH GAG.
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