An Inducible and Reversible Mouse Genetic Rescue System - Genetic Engineering: Recent Developments in Applications - page 252

TIGRE reporter lines TRE-lacZ or TRE-Luc (both with insulators), indicating
that the viral insertion did not disrupt the nearby Carm1 gene.
Figure 5. Chromosomal location of the T1 TIGRE locus. (A) Genomic sequences surrounding the T1 TIGRE
locus. The underlined AAAG sequence was duplicated upon viral integration and the viral TIGRE vector was
inserted exactly in between the duplication. (B) BLAT search of the UCSC Mouse Genome Browser (http://
genome.ucsc.edu/cgi-bin/hgBlat) with genomic sequences (as in (A)) surrounding T1 revealed the localization
of T1 locus to chr9 qA3. This panel is a screen shot of the BLAT search result. The location of the sequences
used for the search is indicated by a vertical bar next to “YourSeq”. The insertion site is in between two genes:
AB124611 and Carm1 (alternative name Prmt4), and does not seem to disrupt either gene.
Ko Lines Produced from an es cell Library Mutagenized by a
Retroviral Vector
KO lines with target genes replaced by rtTA or tTA can be produced by any
gene targeting or insertional mutagenic methods. To implement high-throughput