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macroscopic lung metastases, whereas mice treated with BMSCs expressing sFlt-1
decreased lung metastases. The tumor nodules (> 3 mm) of lung surface were sig-
nificantly decreased in the mice treated with BMSCs expressing sFlt-1 (P < 0.05)
(Figure 2A, B). Lung weight, which correlates with total tumor burden, was also
reduced significantly in the team of mice administered with BMSCs expressing
sFlt-1 (Figure 2C). Booming proliferation of tumor cells and severe destruction
of lung tissues were observed in the H.E. staining for control mice, whereas in the
mice treated with BMSCs expressing sFlt-1, cancerous cells were less nourished
and lung tissues were relatively normal (Figure 2D). Benefited from the decrease
of tumor burden in the lung metastases, the lifespan of the tumor-bearing mice
was prolonged significantly (Figure 2E, P < 0.05). The similar results were also
observed from mouse LLC metastasis model (Figure 3).
Figure 2. Anti-tumor effect in mouse CT26 colon adenocarcinoma model. Female BALB/c mice received i.v.
injection of 2×10 5 CT26 cells in 100 µ l of PBS. Treatment with 1×10 6 Adv-GFP-sFlt-1 infected BMSCs (black
rhombus), 1×10 6 Adv-GFP infected BMSCs ( , black quadrilateral), 1×106 uninfected BMSCs (black triangle)
and 100 µ l of 0.9% NaCl solution alone (black circularity) were administered i.v. from tail vein. Treatment
with sFlt-1-bearing BMSCs could decrease the number of and growth of surface metastases (Figure 2-A, D) and
abolish the tumor burden by rendering the weight of lungs similar to that of normal mice (Figure 2-C). Mean
numbers of lung tumor nodules in each group are shown (Figure 2-B). Values are plotted as means ± SEM (P <
0.05). The percentage of metastatic foci > 3 mm are marked as solid bars. A significant increase of survival rate
in sFlt-1-bearing BMSCs treated mice, compared with the controls (Figure 2-E, P < 0.05. By log-rank test), was
found in the tumor model.
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