Biology Reference
In-Depth Information
Table 2. Serial hematocrit measurements in rats after MI, showing that EPO-transduced cell injection does not
increase systemic hematocrit levels
Figure 2. Photomicrographs of heart sections, immunostained with anti-human erythropoietin antibodies
(brown), 4 weeks after cell transplantation. (A) Human kidney positive control. (B) saline-injected heart. (C)
Non-transduced fibroblast-transplanted heart. (D) RhEPO in transduced fibroblast-transplanted heart (infarct
zone). Arrows show rhEPO expression.
The degree of vascularization was evaluated by counting capillary blood ves-
sels in the infarct border zones using α -smooth muscle actin and lectin stainings
for vascular endothelium localization. Compared with non-transduced fibroblasts
and saline, implanted rhEPO-producing fibroblasts enhanced angiogenesis, as
shown in both α -smooth muscle actin (Figure 3A; 15.27 ± 0.37 versus 9.93 ±
0.97 versus 9.57 ± 0.72 vessels/mm2, respectively, p < 0.05), and lectin stained
sections (Figure 3B; 70.1 ± 8.5 versus 47.5 ± 5.9 versus 20.7 ± 3.6 vessels/mm2,
respectively, p < 0.05).
Search WWH ::




Custom Search