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by morhpometric analysis, as previously described [23]. The following parameters
were measured: LV maximal diameter (mm), average wall thickness (mm; aver-
aged from three measurements of septum thickness), average scar thickness (mm;
averaged from three measurements of scar thickness), relative scar thickness (aver-
age scar thickness/average wall thickness), LV muscle area (mm
2
; including the
septum), LV cavity area (mm
2
), whole LV area (mm2), infarct expansion index
([LV cavity area/Whole LV area]/Relative scar thickness), epicardial scar length
(mm), and endocardial scar length (mm).
statistical Analysis
All values are given as means ± standard error of the mean (SEM) from at least
three independent experiments. RhEPO activity results were compared with Stu-
dent's t-test for unpaired data. Cardiomyocyte or general cell apoptosis results
and blood vessel counts were compared by one-way ANOVA with Bonferroni
post test.
In the echocardiography study, because each animal was used as its own con-
trol, changes between baseline and 4 weeks in the control and treated groups were
assessed with paired t-tests. Comparisons of the changes from baseline to 5 and
9 weeks in the control and treatment groups were made with repeated-measure
two-way ANOVA. The ANOVA model included control versus treatment and
baseline versus 5 and 9 weeks as factors, as well as the interaction between the
two factors. GraphPad Prism version 4.00 for Windows (GraphPad Software, San
Diego, California, USA) was used for analysis.
Comparison of morphometry parameters and hematocrit measurements
among treatment groups was analyzed by one-way ANOVA with Bonferroni post
test. All tests were performed using GraphPad Prism Version 4.0. P < 0.05 was
considered statistically significant.
results
Ex Vivo Retroviral Gene Transfer and Rhepo Expression
To test retroviral ability for stable expression of biologically active rhEPO, the
initial transduction experiments were carried out using BALB/3T3 mouse fibro-
blast cell line. Table 1 describes the activity of EPO produced from transduced
cells. ELISA was used to evaluate EPO concentrations, and its biological activity
was determined by proliferation of the EPO-sensitive human erythroleukemia
cell line TF-1. The activity induced by recombinant human EPO (Eprex) was
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