Biology Reference
In-Depth Information
Extraction of the lipophilic phase (LE) was made on pellet by adding acetone
(1:4 w/v), and centrifuging at 2000 × g for 15 min. The supernatant (LE extract)
transferred to vials and stored (-20°C) until assayed. Three extracts were collected
for each sample.
Each extract (HE and LE) was recovered and the antioxidant activities were
measured separately by recording the absorbance at 734 nm in a spectrophotom-
eter (KONTRON Uvikon 941 Plus). Data are expressed as AA induced by the
hydrophilic and lipophilic components, and total antioxidant activity by the sum
of the two phases
statistical Analysis
All the data were subjected to ANOVA according on a Latin square scheme with
4 replicates, Duncan test (p < 0.05) was used for comparison of means.
Authors' contributions
GLR and NA selected the genetic material and coordinated the field trial; ES and
BM were concerned with agronomical work at the field trial; GM, RLS, AM, JS,
BM have performed biochemical and technological analysis; NA and ES have
done the statistical analysis; GLR wrote the manuscript; BM, TP and AS per-
formed the molecular analysis of the plants and the gene constructs used.
Acknowledgements
This work is part of the FIRB project RBAUOIJTHS [23] of the MIUR (Italian
Ministry of University and Research).
references
1. Varoquaux F, Blanvillain R, Delseny M, Gallois P: Less is better: new approach-
es for seedless fruit production. Trends Biotec 2000, 18:233-242.
2. Spena A, Rotino GL: Parthenocarpy: State of the Art. In Current Trends in the
Embryology of Angiosperms. Edited by: Bhojwani SS, Soh WY. The Nether-
lands: Kluwer Academic Publishers; 2001:435-450.
3. Schwabe WW, Mills JJ: Hormones and parthenocarpic fruit set: a literature
survey. Hort Abstracts 1981, 51:661-698.
Search WWH ::
Custom Search