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competing interests
The authors never received reimbursements, fees, funding, or salary from an orga-
nization that may in any way gain or lose financially from the publication of this
paper. The authors never have any stocks or shares in an organization that may in
any way gain or lose financially from the publication of this paper. The authors
have no competing interests to declare in relation to this paper.
Authors' contributions
SD was the main contributor to this paper. This work is part of her PhD project.
She carried out transduction of CD34 + cells, transplantation of animals, PCR for
identification of cells expressing the transgene in vivo, flow cytometry analysis,
WG Have improved assays for transduction of macaque bone marrow CD34 +
cells with SIV derived vector, DN constructed and produced the SIV derived
vector, SP technical assistance to cell sorting, MLD technical assistance to trans-
plantation, BD technical assistance to cell culture, flow cytometry and irradiadion
of NHP, GA technical assistance to molecular biology, TA technical assistance to
flow cytometry and cell sorting, JLL irradiation of animals and dosimetry, FLC
supervises vector design and production, RLG supervisor of SD.
Acknowledgements
We would like to thank M. Ripaux, A. Fort, S. Jacquin, D. Mérigard, P. Pochard,
D. Renault, J. C. Wilks and R. Rioux for excellent technical assistance. This work
was supported by the Agence Nationale de Recherches sur le SIDA (ANRS, Paris,
France), the Centre de Recherches du Service de Santé des Armées Emile Pardé
(CRSSA, La Tronche, France), and the Commissariat à l'Energie Atomique (CEA,
Fontenay aux Roses, France).
references
1. Miller DG, Adam MA, Miller AD: Gene transfer by retrovirus vectors occurs
only in cells that are actively replicating at the time of infection. Mol Cell Biol
1990, 10:4239-4242.
2. Jones RJ, Wagner JE, Celano P, Zicha MS, Sharkis SJ: Separation of pluripo-
tent haematopoietic stem cells from spleen colony-forming cells. Nature 1990,
347:188-189.
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