Reconstitution of the Myeloid and Lymphoid Compartments after the Transplantation of Autologous and Genetically Modified CD34 Bone Marrow Cells, Following Gamma Irradiation in Cynomolgus Macaques - Genetic Engineering: Recent Developments in Applications - page 137

peripheral blood cells. In the three remaining animals, vector DNA was detected
in peripheral blood cells (up to 500 copies per million cells) and in the bone mar-
row (up to 6250 copies per million cells) more than one year after transplantation
(day 471).
Figure 5. Recovery of bone marrow clonogenic activity. Bone marrow-derived colony-forming units following
sublethal irradiation of cynomolgus monkeys transplanted (black bars) or not transplanted with CD34 + cells
(white bars). Mean ± SD of CFC number (triplicate). The results of statistical test are indicates; * indicates a
statistically significant difference (p < 0,0001 (Wilcoxon test)) between day 0 and day 7 for the both group; **
indicates a statistically significant difference (p = 0,0009 (Mann & Whitney test)) at day 43 between animals
undergoing transplantation and those that did not undergo transplantation.
Table 2. Number of DNA copies per million mononuclear cells in peripheral blood (PB) and bone marrow
(BM)
Flow cytometry analysis demonstrated the presence of eGFP-producing cells
among peripheral blood mononuclear cells in myeloid and lymphoid lineges of
monkey 6896 (Figure 6). Peripheral blood cells were sorted on the basis of eGFP