Reconstitution of the Myeloid and Lymphoid Compartments after the Transplantation of Autologous and Genetically Modified CD34 Bone Marrow Cells, Following Gamma Irradiation in Cynomolgus Macaques - Genetic Engineering: Recent Developments in Applications - page 135

4 non irradiated animals, which were used as controls, with a similar bleeding
frequency.
Reconstitution of Hematopoietic Cells in Vivo
Following total-body irradiation with 6 Gy, transfusion and an antibiotic regimen
were required to ensure that all the animals survived. However, one animal from
group 1 (7036) died on day 40 due to profound pancytopenia (Figure 4). This
macaque received the smallest number of autologous and transduced CD34 + BM
cells. All other animals from groups 1 and 2 were studied from days -1 to 471 after
gamma irradiation. Controls were followed over the same period.
Figure 4. Effect of irradiation and transplantation on polymorphonuclear cell, lymphocyte and thrombocyte
counts. All animals were followed during the weeks preceding the study, and for more than 240 days after the
irradiation. We carried out hematological analysis including blood cell counts with an automated hemocytometer
(Coulter Corporation, Miami, USA).
Radiation rapidly induced severe anemia in all animals (data not shown). A
significant decrease in the number of polymorphonuclear cells in the periphery
was observed, starting on day 1 after irradiation (Figure 4). No significant dif-
ference was observed between the animals of groups 1 and 2 in terms of the
minimum number of cells (821 ± 226 cells/µl for group 1 and 658 ± 107 cells/µl
for group 2, P = 0.3768 (Mann & Whitney test)) or the time at which that mini-
mum occurred (6 ± 5 days for group 1 and 7 for group 2, P = 0.4795 (Mann &