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eLisA inhibition test
The ELISA inhibition test showed significant dose-dependent inhibition for wild-
type and GM potato extracts. In addition, the two extracts had similar poten-
cies. By contrast, minimal inhibition was noted using D. pteronyssinus. Figure 2
shows the ELISA inhibition test by the addition of extracts from wild-type and
GM potato and Dermatophagoides pteronyssinus. The same pooled sera from
patients sensitized to potatoes were used (A: wild-type potato; B: GM potato).
Figure 2. Percent inhibition of IgE-ELISA by the addition of: extract from wild-type potato ( ), GM potato
extract ( ), and Dermatophagoides pteronyssinus ( ). Sera from patients sensitized to potatoes were used. A:
wild-type potato; B: GM potato.
sds-PAGe and ige-immunoblot Analysis
The IgE-binding components within the wild-type and GM potatoes were
compared using the sera of eight individuals with high specific IgE levels and
sera pooled from the controls. The latter was derived from 10 patients who
responded negatively to the two potato extracts on the skin-prick test. Three
components (45, 34, and 26 kDa) were noted in >50% and 11 components
(78, 72, 64, 36, 35, 25, 23, 22, 20, 19, and 14 kDa) in 33% of patients
sensitized to wild-type potato. One component (45 kDa) was noted in 88%
and eight components (78, 64, 35, 26, 25, 23, 22, and 14 kDa) in <50% of
patients sensitized to GM potato. Thus, the 45-kDa band present in serum
was the most frequently bound (>80%) by extracts from wild-type and GM
potatoes (Fig. 3).
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