The Establishment of in vitro Model of Primary Rat Brain Microvascular Endothelial Cells - Advances in Computational Science and Engineering - page 113

ability of proliferation than adult ones, it is more likely to succeed if we choose infancy
animals. So, rats younger than 2 weeks could be used, but the assist of microscope to
remove meninges is necessary. And laboratory has attempted to use the experimental
miniature pigs after born about one month as sampling animals, the brain tissue vol-
ume is big, cerebral cortex is plentiful, and the animal body type is comparatively
small, it is relatively easy to draw materials, the cost is cheaper than cattle brain, so the
effect is comparatively satisfying, it is also a comparatively ideal experimental object.
Through the development in the past 30 years, there were several model systems
that were constructed, which included the model of the blood-brain barrier (BBB) in
vitro that is mainly constitute by the brain microvascular endothelial cell. The meth-
ods of the model construction and composition are different from each other, so the
differences also existed in the results that given by different physiological and bio-
chemical characteristics experiments, the model systems also have their own advan-
tages and disadvantages. At present, there is no such particular model is better than
other ones. Therefore, the research on blood-brain barrier usually will use two or
more different models combined together.
Primary monolayer BMEC is an in vitro model system of the BBB for studying the
physiology, pathology, pharmacology, central nervous system of infection, and also
the first step of building the more advanced model of the BBB in vitro . With the
means of isolation continuous improvement, BMEC and this in vitro model system of
the BBB will get more extensive and be more in-depth studied.
Acknowledgments. This work was supported by Guangzhou Key Technology R&D
Program (No.2008Z1-E401 to H. Cao) and the NIH (RO1 AI40635 to S.H. Huang).
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