The Cell-Multilayered Periosteal Sheet —A Promising Osteogenic and Osteoinductive Grafting Material - New Trends in Tissue Engineering and Regenerative Medicine - page 33

Cells BMS: bone marrow stem cells, ADPS: adipose stem cells, iPS: induced pluripotent stem
cell, ES: embryonic stem cells
5.2.2. Beneficial technical challenges
It is definitely possible to achieve remarkable regeneration using cell-based therapy compared
with conventional regenerative treatment for satisfaction of patients and cost benefit aspects.
The matrix and scaffold should have good biocompatibility in terms of the cellular and
molecular components during the process of developing and regenerating tissues. The
synergistic effects of cell-based therapy with suitable scaffolds and growth factors are antici‐
pated and should be substantiated in the near future.
5.2.3. Challenges of cell delivery system and cell banking
Cell processing center/room (CPC) is required for cell-based therapy, and the refinement of
techniques to facilitate laboratory handing of cells is also crucial for biological and clinical
applications. CPC should be established at hospital, however, it is impossible to establish CPC.
Thus, a well-controlled cell delivery system is necessary for wide area of several prefectures.
Furthermore, cell banking may be one strategy to realize the potential of cell-based regenera‐
tive therapy. Several types of cells can be cryopreserved to retain regenerative potential. Dental
cells can be isolated from the cryopreserved tissue whenever required for future regenerative
therapies [Chen et al., 2012].
Author details
Tomoyuki Kawase, Kazuhiro Okuda, Masaki Nagata and Hiromasa Yoshie
Institute of Medicine and Dentistry, Niigata University, Japan
References
[1] Chen FM, Sun HH, Lu H, & Yu Q. (2012) Stem cell-delivery therapeutics for perio‐
dontal tissue regeneration. Biomaterials 33: 6320-6344.
[2] Egusa H, Sonoyama W, Nishimura M, Atsuta I, & Akiyama K. (2012) Stem cells in
dentistry--Part II: Clinical applications. J Prosthodont Res 56: 229-248.
[3] Engler AJ, Sen S, Sweeney HL, & Discher DE. (2006) Matrix elasticity directs stem cell
lineage specification. Cell 126: 677-89.
[4] Horimizu M, Kawase T, Tanaka T, Okuda K, Nagata M, Burns DM, & Yoshie H.
(2013) Biomechanical evaluation by AFM of cultured human cell-multilayered peri‐
osteal sheets. Micron 48: 1-10.