Chemistry Reference
In-Depth Information
tAble 2.3
example Flow rate Accuracy test result Form
Pump Serial Number
Flow Rate Accuracy Results
test results
set Flow
rate
(ml/min)
observed
system
pressure (psi)
observed
elapsed
time (s)
calculated
Flow rate
(ml/min)
Flow rate
speciication
(ml/min)
pass
(Initials)
Fail
(Initials)
5.000
4.94-5.06
1.000
0.988-1.012
0.500
0.489-0.511
0.250
0.239-0.261
Comments
Certification
The Performer as signed below attests that the test was performed according to the prescribed
procedure and that the results above are accurate and complete.
Performer
Signature
Date
The Reviewer as signed below attests that the test was performed according to the prescribed
procedure and that the results above are accurate and complete.
Reviewer
Signature
Date
Note:
Flow rates and specifications are suggestions only, and should be based upon actual use, vendor's
specifications, and measurement error.
injection (assuming six injections were performed between weighings) is calculated
according to the formula:
mg or µL water per injection = 1,000 × [(W
1
− W
2
)/6]
Water is often used for this test because its density, 0.99823 g/mL at 20°C and
0.99707 g/mL at 25°C, introduces less than 0.3% error when volume is assumed
equal to weight (grams × 1,000 = µL).
2.3.3.4 column oven and Auto sampler temperature
The temperature of the sample compartment or the column oven is measured using
a thermometer probe placed so that it does not come into contact with the surface
in either component. A temperature is set that is at least 5°C above or below the
ambient temperature, and sufficient time is allowed for the temperature to stabi-
lize before recording the value. The time required to reach and stabilize the set
temperature depends on the difference between the set and ambient temperatures.
