Biology Reference
In-Depth Information
Fig. 9.1
Placental TauT expression and activity are similar in first trimester and at term. (
a
) TauT
mRNA expression relative to a 40-week placental sample, designated the calibrator, included in
each run as an internal standard. The groups are early first trimester (6-9 weeks,
n
= 23), late fi rst
trimester (10-13 weeks,
n
= 12), and term (38-40 weeks,
n
= 21). (
b
) Western blot of first trimester
(F) and term placental (T) samples probed for TauT. A single immunoreactive species was detected
at the expected molecular weight of 70 kDa. Bar chart displays densitometric analysis of signal
intensity (
n
=4 for each group, median and interquartile range). (
c
) Na
+
-dependent
3
H-taurine
uptake by first trimester (
open circles
) and term (
closed squares
) placental villous fragments (
n
= 6
in each group; mean ± SE). At both gestations,
3
H-taurine uptake significantly increased with time
(
p
< 0.005; least square linear regression). There was no difference in
3
H-taurine uptake between
fi rst trimester and term placenta (2-way ANOVA)
Fig. 9.2
Placental STB TauT activity is negatively related to maternal BMI and reduced in
pre-eclampsia (PE). (
a
) Negative relationship between maternal BMI and TauT activity in first
trimester (
open circles
,
n
= 12) and term (
closed squares
,
n
= 12) placental villous fragments. Least
square linear regression;
r
2
= 0.60 and 0.54, respectively,
p
< 0.01 for each. (
b
) TauT activity in
normal pregnancy (NP,
n
=9) and PE (PE,
n
=5) in women with BMI <30.
p
< 0.01: Mann
Whitney-
U
test
Search WWH ::
Custom Search