Perinatal Taurine Imbalance Alters the Interplay of Renin–Angiotensin System and Estrogen on Glucose–Insulin Regulation in Adult Female Rats - Advances in Experimental Medicine and Biology - page 67

Fig. 8.1 Animal treatment protocol ( CW control with water intake alone, CW + Cap CW plus
captopril treatment, CW + Tam CW plus tamoxifen treatment, CG control with high sugar intake,
CG + Cap CG plus captopril treatment, CG + Tam CG plus tamoxifen treatment, TDW perinatal
taurine depletion with water intake alone, TDW + Cap TDW plus captopril treatment, TDW + Tam
TDW plus tamoxifen treatment, TDG perinatal taurine depletion with high sugar intake, TDG + Cap
TDG plus captopril treatment, TDG + Tam TDG plus tamoxifen treatment, TSW perinatal taurine
supplementation with water intake alone, TSW + Cap TSW plus captopril treatment, TSW + Tam
TSW plus tamoxifen treatment, TSG perinatal taurine supplementation with high sugar intake,
TSG + Cap TSG plus captopril treatment, TSG + Tam TSG plus tamoxifen treatment)

8.2.2 Experimental Protocol

At 7-8 weeks of age, under thiopental anesthesia (50 mg/kg body weight, i.p.), all
female rats were implanted with femoral arterial and venous catheters. Two days
later and after an overnight fasting, arterial blood samples (0.2 ml each) were col-
lected for plasma insulin and fasting blood sugar and replaced with normal saline.
Thereafter, glucose tolerance testing (GTT) was initiated by intravenous injection of
glucose (2 g/kg in saline), and blood glucose levels were immediately measured at
30, 60, and 120 min. At the end of the experiment, all animals were sacrificed by a
lethal dose of thiopental and heart and kidney weights were collected.