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Fig. 8.1
Animal treatment protocol (
CW
control with water intake alone,
CW + Cap
CW plus
captopril treatment,
CW + Tam
CW plus tamoxifen treatment,
CG
control with high sugar intake,
CG + Cap
CG plus captopril treatment,
CG + Tam
CG plus tamoxifen treatment,
TDW
perinatal
taurine depletion with water intake alone,
TDW + Cap
TDW plus captopril treatment,
TDW + Tam
TDW plus tamoxifen treatment,
TDG
perinatal taurine depletion with high sugar intake,
TDG + Cap
TDG plus captopril treatment,
TDG + Tam
TDG plus tamoxifen treatment,
TSW
perinatal taurine
supplementation with water intake alone,
TSW + Cap
TSW plus captopril treatment,
TSW + Tam
TSW plus tamoxifen treatment,
TSG
perinatal taurine supplementation with high sugar intake,
TSG + Cap
TSG plus captopril treatment,
TSG + Tam
TSG plus tamoxifen treatment)
8.2.2
Experimental Protocol
At 7-8 weeks of age, under thiopental anesthesia (50 mg/kg body weight, i.p.), all
female rats were implanted with femoral arterial and venous catheters. Two days
later and after an overnight fasting, arterial blood samples (0.2 ml each) were col-
lected for plasma insulin and fasting blood sugar and replaced with normal saline.
Thereafter, glucose tolerance testing (GTT) was initiated by intravenous injection of
glucose (2 g/kg in saline), and blood glucose levels were immediately measured at
30, 60, and 120 min. At the end of the experiment, all animals were sacrificed by a
lethal dose of thiopental and heart and kidney weights were collected.
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