Biology Reference
In-Depth Information
has been shown to rescue the effects of bisphosphonate-mediated inhibition on
cellular signaling (Andela et al.
2003
), thereby providing a mechanism to demon-
strate whether the bisphosphonates utilized the mevalonate pathway in the inhibi-
tion of secreted VEGF in retina. In our result, increased [
3
H]taurine uptake by
pretreatment of alendronate and pamidronate was significantly reduced by GGOH
in TR-iBRB cells under high glucose condition (Fig.
7.3
).
7.5
Conclusion
In conclusion, taurine transport through the iBRB under high glucose condition can
be regulated by bisphosphonates via mevalonate pathway. Therefore, we suggest
that bisphosphonates could have the beneficial effects on DR by regulation of
taurine contents in retina.
Acknowledgements
This work was supported by the Research Grant of Sookmyung Women's
University.
References
Andela VB, Pirri M, Schwarz EM, Puzas EJ, O'Keefe RJ, Rosenblatt JD, Rosier RN (2003) The
mevalonate synthesis pathway as a therapeutic target in cancer. Clin Orthop Relat Res
415(Suppl):S59-S66
Ando D, Kubo Y, Akanuma S, Yoneyama D, Tachikawa M, Hosoya K (2012) Function and regula-
tion of taurine transport in Müller cells under osmotic stress. Neurochem Int 60:597-604
El-Sherbeny A, Naggar H, Miyauchi S, Ola MS, Maddox DM, Martin PM, Ganapathy V, Smith
SB (2004) Osmoregulation of taurine transporter function and expression in retinal pigment
epithelial, ganglion, and Müller cells. Invest Ophthalmol Vis Sci 45:694-701
Frank RN (1984) On the pathogenesis of diabetic retinopathy. Ophthalmology 91:626-634
Frank RN (2004) Diabetic retinopathy. N Eng J Med 350:48-58
Geggel HS, Ament ME, Heckenlively JR, Martin DA, Kopple JD (1985) Nutritional requirement
for taurine in patients receiving long-term parenteral nutrition. N Engl J Med 312:142-146
Gilbert RE, Vranes D, Berka JL, Kelly DJ, Cox A, Wu LL, Stacker SA, Cooper ME (1998)
Vascular endothelial growth factor and its receptors in control and diabetic rat eyes. Lab Invest
78:1017-1027
Heller-Stilb B, van Roeyen C, Rascher K, Hartwig HG, Huth A, Seeliger MW, Warskulat U,
Häussinger D (2002) Disruption of the taurine transporter gene (taut) leads to retinal degenera-
tion in mice. FASEB J 16:231-233
Hosoya K, Tomi M, Ohtsuki S, Takanaga H, Ueda M, Yanai N, Obinata M, Terasaki T (2001)
Conditionally immortalized retinal capillary endothelial cell lines (TR-iBRB) expressing
differentiated endothelial cell functions derived from a transgenic rat. Exp Eye Res 72:
163-172
Jacobsen JG, Smith LH (1968) Biochemistry and physiology of taurine and taurine derivatives.
Physiol Rev 48:424-511
Luckman SP, Hughes DE, Coxon FP, Russell GG, Rogers MJ (2005) JBMR anniversary clas-
sic. Nitrogen-containing biophosphonates inhibit the mevalonate pathway and prevent
Search WWH ::
Custom Search