Effect of Taurine Feeding on Bone Mineral Density and Bone Markers in Rats - Advances in Experimental Medicine and Biology - page 50

Table 6.1 Composition of experimental diets (g /1 kg diet)
Dietary group
Control Taurine
Corn starch 531 531
Sucrose 100 100
Casein a 200 200
Soybean oil 70 70
Cellulose b 50 50
Mineral-Mix c 35 35
Vitamin-Mix d 10 10
L -Cystein e 1.8 1.8
Choline f 2.5 2.5
TBHQ g 0.008 0.008
Taurine h 0 20
a Casein, Maeil Dairy industry Co. Ltd. 480 Gagok-Ri, JinwiMyun, Pyungtaek-City, Kyunggi-Do,
Korea
b a-Cellulose, Sigma Chemical Co., St. Louis, Mo, USA
c Mineral-mix, AIN-93 G-Mx, Teklad Test Diets, Medison, Wisconsin, USA
d Vitamin-mix, AIN-93-VM, Teklad Test Diets, Medison, Wisconsin, USA
e L-Cystine, Sigma Chemical Co., St. Louis, MO, USA
f Choline bitartate, Sigma Chemical Co., St. Louis, MO, USA
g Tert-bultyl hydroquione, Sigma-Aldrich Inc., St. Louis, MO, USA
h Taurine, Dong-A Pharm Co. Ltd. 434-4 Moknae-dong. Ansan-City
and their food intake and body weights were measured every other day and once a
week, respectively. The rats were fed a AIN-96 control diet and control plus taurine
diet (2% wt/wt) for 6 weeks. Compositions of the diets were as described in
Table 6.1 . Blood samples were collected from the abdominal aorta and serums were
separated at 3,000 rpm for 20 min. Serums were stored at −70°C until analysis.
Serum concentrations of alkaline phosphatase (ALP) and osteocalcin were
measured. Serum calcium and phosphate were also measured. Serum ALP and
osteocalcin and urinary deoxypyridinoline (DPD) cross-link values were measured
as markers of bone formation and resorption. ALP activity was reported as units per
liter (U/L). The concentration of urine DPD was measured with an enzyme immu-
noassay that preferentially recognizes the free form of DPD (CLIA, Pyrilinks-D
DPC, USA). DPD was corrected for creatinine excretion (DPD/Cr). The concentra-
tion of serum osteocalcin was measured with an osteocalcin kit (IRMA, OSTEO-
RIACT, Cis Bio, Saclay, France), which recognizes the intact form of osteocalcin.
Serum ALP activity was measured with a kit from Enzymatic assay (Prueauto S
ALP) following the manufacturer's instructions.
BMD and BMC were measured using PIXImus (GE Lunar Co, Wisconsin, USA)
in spine and femur on 6 weeks after feeding. The experimental protocol was approved
by Institutional Animal Care and Use Committee (IACUC) in Keimyung University
and conformed to the Guide for the Care and Use of Laboratory Animals.