The Mechanism of Taurine Protection Against Endoplasmic Reticulum Stress in an Animal Stroke Model of Cerebral Artery Occlusion and Stroke-Related Conditions in Primary Neuronal Cell Culture - Advances in Experimental Medicine and Biology - page 234

Fig. 23.3 Effect of taurine on ATF6 and p-IRE1 pathway in neuronal cell cultures under
hypoxia/reoxygenation condition and in rat MCAO stroke model. Taurine does not alter activity
of the PERK pathway, but inhibits the ATF6 and IRE1 pathway after hypoxia/reoxygenation and
stroke. Norm normoxia, Hyp hypoxia (0.3% O 2 ) for 20 h, reoxygenation for 20 h, Ta u + Hyp
neurons were treated with 10 mM taurine for 1 h, then hypoxia for 20 h, reoxygenation for 20 h,
MCAO middle cerebral artery occlusion for 2 h followed by 4 days reperfusion, MCAO + taurine
middle cerebral artery occlusion for 2 h followed by 4 days reperfusion, taurine was injected
24 h after reperfusion subcutaneously and injection continued for 4 days. ( a ) ATF6 expression
in primary neuronal culture analyzed by Western blot. ( b ) ATF6 expression in the core of MCAO
brain analyzed by Western blot. The bar graphs represent the ratio of cleaved ATF6 to ATF6
using the densitometric data from the experiment of ATF6 Western blot results with arbitrary
units. ( c ) P-IRE1 expression in primary neuronal culture analyzed by Western blot. ( d ) P-IRE1
expression in the core of MCAO brain analyzed by Western blot. The bar graphs re fl ect the
densitometric data from the experiment of P-IRE1 Western blot results with arbitrary units. The
values in bar graph represent mean ± SEM, n = 3, ** p < 0.01 versus norm; ## p < 0.01 versus hyp
or MCAO