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protective roles in heart under various pathophysiological conditions (Allard et al.
2006
; YJ et al.
2008
), the mechanism remains to be unclear.
In the present study, the preventive effects of taurine on heart coefficient;
ultrastructure of cardiac muscle; the levels of CK-MB, cAMP, and cGMP; and
antioxidant ability in cardiac hypertrophy rats induced by isoproterenol were
investigated.
21.2
Methods
21.2.1
Experimental Animals and Treatments
Male Wistar rats (weighted 250 g ± 30 g) were obtained from the animal center of
development zone in Changchun, China (Certificate No 2003-0004). They were
maintained in controlled light (14 h light, 10 h dark) and temperature (22 ± 2°C) and
were given free access to rat chow diet and tap water. After acclimatizing for 3 days,
45 rats with normal electrocardiogram were randomly divided into five groups, nine
in each group. In control group, rats were administered with physiological saline by
subcutaneous (s.c.) injections. Isoprenaline (ISO) at a dosage of 10 mg (dissolved in
physiological saline)/kg d was s.c. administered to rats of the model group for 7
days. The other groups were the taurine and ISO co-treated; rats were administered
with 100 mg/kg d (preventive group I, PI), 200 mg/kg d (preventive group II, PII),
and 300 mg/kg d (preventive group III, PIII) taurine, respectively, by intraperitoneal
(i.p.) injection 30 min before injection of ISO. The experiment was lasted for 7 days.
21.2.2
Chemicals
Taurine and isoprenaline were purchased from Sigma Chemical Company (St.
Louis, MO, USA). Reagent kits of total protein (TP), reduced glutathione (GSH),
superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase
(GSH-Px), total antioxidation capacity (T-AOC), a creatine kinase MB (CK-MB)
Elisa (ADL, USA), cyclic adenosine monophosphate (cAMP) Elisa (ADL, USA),
and cyclic guanosine monophosphate (cGMP) Elisa (ADL, USA) were purchased
from Nanjing Jiancheng Bioengineering Institute (CHINA).
21.2.3
Heart Coef fi cient
On the 8th day, rats were sacrificed after being fasted overnight; the hearts were
quickly excised; atrium cordis and connective tissue were removed, rinsed with
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