Biology Reference
In-Depth Information
Chapter 17
Antioxidant and DNA Protection Effects
of Taurine by Electron Spin Resonance
Spectroscopy
Sun Hee Cheong , Sang Ho Moon , Seung Jae Lee , Sung Hoon Kim ,
and Kyung Ja Chang
Abstract Taurine may play an important role in protecting cells against toxic injury by
an antioxidant. However, there is a lack of evidence to support this hypothesis. The
objective of this study was to examine the in vitro antioxidant properties of taurine
against different reactive species at various concentrations. The radical scavenging
effects of taurine on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical,
superoxide radical, and alkyl radical were investigated using a spin-trapping electron
method and compared with the electron spin resonance (ESR) signal intensity. ESR
assays showed that DPPH radical scavenging activity of taurine at various concentra-
tions (0.0625~1 mg/mL) was elevated with a decrease of ESR signals in a dose-
dependent manner. Moreover, taurine exhibited the radical scavenging activities against
Sun Hee Cheong and Sang Ho Moon contributed equally to this work.
S. H. Cheong ( * )
Department of Applied Biological Chemistry,
Tokyo University of Agriculture and Technology,
Fuchu, Tokyo 183-8509 , Japan
Department of Food and Nutrition, Inha University,
Incheon 402-751 , South Korea
e-mail: sunheecheong@gmail.com
S. H. Moon
Department of Food Bio Science, College of Natural Sciences,
Konkuk University , Chungju, Chungchongbuk-do 380-701 , South Korea
S. J. Lee
Department of Biotechnology, Division of Life Science, Konkuk University, College of
Biomedical and Health Science , Chungju, Chungchongbuk-do 380-701 , South Korea
S. H. Kim
Department of Chemistry , Konkuk University , Seoul 143-701 , South Korea
K. J. Chang
Department of Food and Nutrition , Inha University , Incheon 402-751 , South Korea
e-mail: kjchang@inha.ac.kr
Search WWH ::




Custom Search