Agriculture Reference
In-Depth Information
CHAPTER
7
A ROLE FOR HIGH-RESOLUTION MASS SPECTROMETRY
IN THE HIGH-THROUGHPUT ANALYSIS AND
IDENTIFICATION OF VETERINARY MEDICINAL
PRODUCT RESIDUES AND OF THEIR METABOLITES IN
FOODS OF ANIMAL ORIGIN
ERIC VERDON, DOMINIQUE HURTAUD-PESSEL, and
JAGADESHWAR-REDDY THOTA
7.1
INTRODUCTION
The analysis of residues of veterinary medicinal products (VMPRs) is of growing
interest due to their impact on human health [1,2]. For public health reasons, it is
necessary to ensure that food products of animal origin are free from VMPR
contamination or that they are safe in terms of public health before bringing them
to the market. In today
s global market, concerns related to food safety are becoming
increasingly important. Across the world, many countries are rigorously following
scienti
'
cally well-established, biological and physicochemical methods to determine
VMPRs in biological sample matrices. The physicochemical analyses of drug
residues (from medicinal products) or contaminants (toxins and organic pollutants)
are currently performed using mainly molecular separation techniques (chromatog-
raphy) involving the collection of spectral information. However, as indicated in
Chapter 2, there has recently been a rapid advancement in the use of mass
spectrometry, particularly coupled with liquid chromatography (LC) [3,4]. For
example,
liquid chromatography with triple quadrupole(QqQ) mass analyzers
(LC
QqQ) are widely used in the food testing laboratories across
the world for qualitative and quantitative analyses of targeted veterinary drug residues
at sub-ng/g level that are present in complex biological matrices. Preselected targeted
analysis bene
-
MS/MS or LC
-
ts from the high sensitivity and selectivity of tandem mass spectrome-
try systems operated in the multiple reaction monitoring (MRM) or selective reaction
monitoring (SRM) modes. However, these modes have some limitations: (i) The
screening of targeted analytes above the instrument capacity in a single run is not
feasible. Additionally, it needs the availability of all targeted standards to record the
MRM values and conditions. (ii) The detection of unexpected/untargeted compounds
