Agriculture Reference
In-Depth Information
complementary and they showed their combination as a powerful tool for analysis of
macrolide residues and their degradation products in food matrices.
Hermo et al. [98] developed an HPLC
MS method for the determination and
characterization of eight quinolones in liver, which was subsequently compared
with both HPLC
-
TOF
-
-
Q
-
MS and HPLC
-
QqQ
-
MS/MS methods. The obtained LOQs
were 1
-
4 times higher using Q
-
MS than TOF
-
MS, whereas the LOQs were 1.5
-
6
times higher using TOF
-
MS than QqQ
-
MS/MS, and thus, sensitivity followed this
order: QqQ
TOF
Q. It must be noted that the LOQs obtained with TOF
-
MS were
>
>
suf
cient to detect quinolones below the EUMRLs. On the other hand, CC
α
and CC
β
values were comparable to those obtained using Q
-
MS or QqQ
-
MS/MS. Despite
these results, the authors highlighted the advantage of the LC
MS system to
provide high-quality data by exact mass measurement. These results indicated that
TOF
-
TOF
-
rmatory
method in the EU legislative context. These three analyzers were employed to analyze
different pig liver samples and only one sample contained enro
-
MS can be considered a very compelling instrument for use as a con
oxacin, detected with
the three approaches (Figure 6.4). When this sample was quanti
ed, the concentration
Figure 6.4.
Chromatograms obtained for enrofloxacin in a sample of liver by (a) LC-Q-MS, (b) LC-QqQ-
MS/MS, and (c) TOF-MS.
Source
: Ref. [98], Figure 6, p. 13. Reproduced with permission of Elsevier.
