Chemistry Reference
In-Depth Information
consisting of cell-adhesive and cell-repulsive regions are investigated, which allow
to control cell position, migration, proliferation, phenotype, membrane integrity, and
finally life and death [247]. These patterns can be generated completely by plasma-
based processes [248-252]. They are sometimes called
plasma lithography
[252].
Special advantages of plasma processes in this context are a good reliability and
potential for facilitated integration in industrial manufacturing processes [253]. Such
plasma-generated micro patterns [254] are depicted in Figure 8.39.
Topics where chemical micropatterning of biomaterial surfaces is investigated
are, for instance, the development of bioartificial neuronal networks [255], advanced
cell culture systems for a bioartificial liver in sandwich structure [256], for DNA-
and proteinchips in high-throughput screening [257], and for high-content screening
[246]. A triple chemical micropattern was described in [246] for the purpose of
transverse small interfering ribonucleic acid (siRNA) transfection. This technique
requires the combination of three differing types of surface adhesiveness on a chip,
(a)
(b)
(c)
FIGURE 8.39
Plasma-generated chemical micropattern (structured width 100 μm):
(a) qualitative chemical characterization by imaging O1s XPS signal. (From Schröder, K.
et al.,
Surf. Interface Anal.
, 36, 702, 2004.) (b) visualization by fluorescence labeling (CY3)
of amino groups, and (c) pattern-guided growth of nasopharyngeal epithelial cells on lat-
eral chemical microstructures. (From Schröder, K. and Ohl, A., Plasmamodifikationen und
Proteinkopplungen für biomedizinische Anwendumgen, in
Proceedings 13, Neues Dresdner
Vakumtechnisches Kolloquium
, Dresden, Germany, pp. 42-49, 2005.)
