Chemistry Reference
In-Depth Information
concentration of C
sat
E
8.8 kg m
3
of b-lactoglobulin would be required to
saturate the surfaces of emulsion droplets coated with lactoferrin. Similarly, a
minimum concentration of C
sat
E
14 kg m
3
of lactoferrin would be needed to
saturate the surfaces of emulsion droplets formed with b-lactoglobulin.
Oil-in-water emulsions containing oil droplets surrounded by multilayered
interfacial layers consisting of low-molecular-weight emulsifiers (SDS or leci-
thin) and biopolymers (gelatin, chitosan, pectin) have been studied exten-
sively.
12-14
These systems tend to show extensive bridging flocculation upon
charge neutralization when biopolymers with an opposite charge from that of
the droplets are added to the emulsion. In contrast, the multilayered emulsion
droplets formed here using b-lactoglobulin + lactoferrin appear to be stable to
droplet aggregation even at very low net electrical charges, presumably because
of strong steric repulsion associated with the protein interfacial layers. Further
work is underway to determine the behaviour of these emulsions under different
environmental conditions, such as pH, temperature and ionic strength.
Acknowledgements
We gratefully acknowledge financial support from the New Zealand Founda-
tion for Research, Science and Technology. We thank Dandan Chen, Jeniene
Gilliland and Michelle Tamehana for assistance with the experimental work.
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