Biomedical Engineering Reference
In-Depth Information
9.
The PCR primers target 50 700-bp amplicons that overlap with each other along
the SARS-CoV genome. The sequencing primers are designed in such a way that
any sequence masked over by the PCR primer binding sites and the sequencing
primer peak on one amplicon are reliably backed up by the homologous sequence
in the overlapping amplicon.
10.
We advocate scrutinizing efforts in validating any genomic sequence variation
by resequencing regions with different combination of primers and sequencing
chemistry. Because variation seen in a single viral isolate could potentially be a
result of sequencing artifacts, we consider only the genomic sequence variations
that are shared by at least two SARS-CoV isolates.
Acknowledgments
This work is supported by a Special Grant for SARS Research (CUHK 4508/
03M) from the Research Grants Council of the Hong Kong Special Adminis-
trative Region (China).
References
1. Poutanen, S. M., Low, D. E., Henry, B., et al. (2003) Identification of severe acute
respiratory syndrome in Canada.
N. Engl. J. Med.
348,
1995-2005.
2. Lee, N., Hui, D., Wu, A., et al. (2003) A major outbreak of severe acute respira-
tory syndrome in Hong Kong.
N. Engl. J. Med.
348,
1986-1994.
3. Tsang, K. W., Ho, P. L., Ooi, G. C., et al. (2003) A cluster of cases of severe acute
respiratory syndrome in Hong Kong.
N. Engl. J. Med.
348,
1977-1985.
4. Rota, P. A., Oberste, M. S., Monroe, S. S., et al. (2003) Characterization of a
novel coronavirus associated with severe acute respiratory syndrome.
Science
300,
1394-1399.
5. Marra, M. A., Jones, S. J., Astell, C. R., et al. (2003) The genome sequence of the
SARS-associated coronavirus.
Science
300,
1399-1404.
6. Ksiazek, T. G., Erdman, D., Goldsmith, C. S., et al. (2003) A novel coronavirus
associated with severe acute respiratory syndrome.
N. Engl. J. Med.
348,
1953-1966.
7. Drosten, C., Gunther, S., Preiser, W., et al. (2003) Identification of a novel
coronavirus in patients with severe acute respiratory syndrome.
N. Engl. J. Med.
348,
1967-1976.
8. Peiris, J. S., Chu, C. M., Cheng, V. C., et al. (2003) Clinical progression and viral
load in a community outbreak of coronavirus-associated SARS pneumonia: a pro-
spective study.
Lancet
361,
1767-1772.
9. Ng, E. K. O., Hui, D. S. C., Chan, K. C. A., et al. (2003) Quantitative analysis and
prognostic implication of SARS coronavirus RNA in the plasma and serum of
patients with severe acute respiratory syndrome.
Clin. Chem.
49,
1976-1980.
10. Ng, E. K. O., Ng, P. C., Hon, K. L., et al. (2003) Serial analysis of the plasma
concentration of SARS coronavirus RNA in pediatric patients with severe acute
respiratory syndrome.
Clin. Chem.
49,
2085-2088.
