Introduction to the Polymerase Chain Reaction - Clinical Applications of PCR - page 8

11.6. Mass Spectrometry
The PCR products can be analyzed by matrix-assisted laser desorption/ion-
ization time-of-flight mass spectrometry. The PCR products are subjected to a
separate primer extension reaction. The extension products are dotted on a chip
and ionized by laser. The molecular mass of the extension products can then be
determined by the time required to reach the detector. This detection method
has been applied for high-throughput single nucleotide polymorphism
genotyping ( 23-25 ) , mutation detection ( 26 ) , and prenatal diagnosis ( 27 ) .
12. Conclusion
The versatility of PCR has made it one of the most widely used methods in
molecular diagnosis. The number of PCR-based applications has continued to
increase rapidly and has impacted oncology ( see Chapters 4, 10, and 13),
genetics ( see Chapters 8 and 12), and pathogen detection ( see Chapters 14-16). In
Clincial Applications of PCR: Second Edition , we attempt to present some of
the most important clinical applications of PCR.
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