Biology Reference
In-Depth Information
pension cultures. Adherent cultures in roller bottles and suspension cultures in
stirred tank vessels are shown in Figure 7 and Figure 8, respectively.
Commercial cell lines are designed and selected for constitutive production;
therefore, the amount of rHuEPO produced correlates with the viable cell
number (Fig. 9). For adherent culture, the number of cells is limited by surface
area. To increase production of rHuEPO, the surface area is increased, typi-
cally by increasing the number of roller bottles or microcarrier beads. Since
nutrients are supplied in the liquid phase and products are secreted (by the
cells) from the solid phase into the liquid phase, the successful mass transfer
Figure 7. Production of rHuEPO in adherent culture. The left panel shows roller bottle cultures of
rHuEPO. The right panel shows large racks containing many of the same type of roller bottle cultures.
Figure 8. Production of rHuEPO in suspension culture. A suspension of rHuEPO-producing cells is
shown in the spinners on the left. The stirred tank reactor shown on the right also contains a suspen-
sion of rHuEPO-producing cells.
