Biology Reference
In-Depth Information
Figure 4. Schematic of a bioreactor operating in fed-batch mode. The sterile envelope, depicted by the
dotted line, includes the media tank and the bioreactor. The harvest operation, which includes cell sep-
aration from the conditioned medium and concentration of the conditioned medium, is not performed
under sterile conditions.
options and the relative scale of tanks and production vessels is depicted. For
batch and fed-batch processes, the production vessel is typically the largest
piece of equipment used in the cell culture process; for perfusion processes,
the feed and harvest tanks are typically the largest equipment used.
Figure 5. Schematic of a bioreactor operating in perfusion mode. The sterile envelope, depicted by the
dotted line, includes the media tank, bioreactor, cell separation device (harvest unit), and harvest tank.
The harvest operation here does not include any concentration of the conditioned medium.
