Biomedical Engineering Reference
In-Depth Information
The radical rebound mechanism has been proposed and proved in several cases in
reaction of hydroxylation catalyzed by cytochrome 450 and methane monooxigenase
(Section 3
.
2)
2.11. Substrate channeling
Substrate channeling is a process by which two or more sequential enzymes in a pathway
interact to transfer a metabolite (or intermediate) from one enzyme to another without
allowing free diffusion of the metabolite into bulk solvent. (Ovadi, 1991; Srere, 1987;
Anderson, 1999). The substrate tunneling is one of fundamental process of regulating
enzymatic processes in cells. Glycolysis, biosynthesis of nucleic acids, aminoacids, and
fatty acids are found to be among these processes.
Techniques for demonstrating channeling behavior include enzyme buffering, ligand
exchange kinetics, isotope dilution, and estimation of the transition time. One of the most
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