Biomedical Engineering Reference
In-Depth Information
The last two recent decades have been marked by attempts to directly detect directly
and characterize reactive oxidizing species using physico-chemical methods. During the
reaction of m-chloroperbenzoate with the low spin ferric form of cytochrome
the formation of several transient intermediates was detected by employing rapid scan
absorption spectroscopy (Egava et al., 1994). The first one appearing within 10 ms gave
an adsorption spectrum similar to those of compound I of chloroperoxidase, another
thiolate-heme protein. In contrast, Blake and Coon (1981) in their spectral and kinetic
experiments on interaction of cytochrome P450LM2 with various peroxycompounds, did
not detect intermediates which can be attributed to the compound II or I. In an attempt to
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