Biology Reference
In-Depth Information
Figure 19.1. Dot plot analysis showing the heterogeneous and peculiar forward and side light
scatter properties of peritoneal cells recovered from Hu-PBL-SCID mice at 4 wks after recon-
stitution. Human cells appear as the relevant lymphoid population evidenced in RI region.
enclosed in mouse fat. It is generally preferable to collect a certain number of
mouse lymph nodes and to pool them for subsequent analysis.
In experiments of HIV-1 infection of hu-PBL-SCID-mice, spleen and lymph
nodes can generally be used for polymerase chain reaction (PCR) analysis and/
or co-cultivation assays with phytohemagglutinin ( PHA)-stimulated PBLs to
monitor p24 release and recover infectious virus.
RECIPROCAL HOST±GRAFT REACTIONS AND DYNAMICS OF
HUMAN PBL ENGRAFTMENT
SCID mice generally do not reject allogeneic or xenogeneic organ grafts and
represent a unique model for investigating in vivo the behavior of both normal
and neoplastic human cells ( Bosma and Carroll, 1991; Mosier, 1996). However,
xenogeneic transplantation in SCID mice may fail completely because of the
murine natural immune reaction (Zubair et al., 1996). In fact, SCID mice retain
fully functioning and highly e½cient natural killer (NK) cells, macrophages,
and granulocyte compartments ( Bosma et al., 1991). Studies aimed at improv-
ing human cell engraftment have shown that control of the natural immune
reactions through the injection antibodies against either NK cells (Barry et al.,
1991; Habu et al., 1981; Lacerda et al., 1996) or granulocyte antigens (Santini
et al., 1998) can improve human cell engraftment. We reported that the major
reaction of SCID mice to the engraftment of human PBL was a massive gran-
ulocyte recruitment into the site of transplantation (Santini et al., 1995). In fact,
FACS analysis of the cells recovered from mouse peritoneum 2 h after engraft-
ment revealed an increase in murine H2K
d
cells, which were speci®cally stained
