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F i g u r e 15.3. (A and B) Freshly stained tetramer-positive cells lack constitutive expression of IFN-g. The ®rst panel set shows the isotype staining control.
(C ) Expansion of tetramer-positive cells in response to Gag peptide stimulation and increased expression of intracytoplasmic IFN-g and surface expression of
CD25 (low-a½nity IL-2R). Increased expression is in relation to Gag-speci®c CTL lysis from 0% in fresh PBMC to a mean of 42.9% after 14 days of culture.
