The Use of Peptide/MHC Tetramers to Visualize, Track, and Characterize Class I-Restricted Anti-HIV T-cell Responses - Cellular Aspects of HIV Infection - page 309

Figure 15.1. (A) The principle of peptide/MHC tetramer staining in a mixture of CD8 T cells
expressing di¨erent T-cell receptor (TCR) speci®cities. This example shows an HLA-A*0201 tet-
ramer binding to the TCR recognizing and epitope in Gag. FACS analysis shows gating around
high CD8 antigen expression to determine tetramer positivity and surface antigen expression. (B)
Expression of Gag- and Pol-speci®c CD8 cells from fresh PBMC in one representative HIV-1 in-
fected individual with concomitant lack of peptide-speci®c CTL lysis.