Biology Reference
In-Depth Information
F i g u r e 12.1. Inappropriate priming for apoptosis of peripheral lymphocytes from HIV-infected
persons. PBMC from control donors or HIV
persons were analyzed ex vivo for the expression of
activation markers (A) or the susceptibility for apoptosis induced by overnight incubation in
medium (spontaneous) (B, C ), anti-CD3 monoclonal antibodies (mAbs) (B), anti-CD95 mAbs ( B)
or anti-TNF-RI mAbs ( E ). (A) The expression of the indicated activation markers has been ana-
lyzed by ¯ow cytometry on PBMC from controls, stage II or stage IV untreated HIV-infected per-
sons, and the data represent the mean percentage of CD4 T cells expressing the corresponding
activation marker within total CD4 T cells. p Values indicate signi®cant di¨erences compared with
control donors. (B) PBMC from the same subjects have been cultured overnight in medium or
having been coated with anti-CD3 mAbs. Apoptosis was measured using the 7-AAD staining, as
described (Gougeon et al., 1996). The mean percentage of apoptotic cells within the CD4 T-cell
subset is shown. (C ) Spontaneous apoptosis (overnight incubation in medium) within the CD4
subset has been followed in three patients over 4 years. The clinical stages of the patients are in-
dicated and for patients CNA, the arrow shows the progression from stage II to stage IV C2. (D)
Anti-CD95-induced apoptosis has been measured in PBMC from a series of nontreated patients,
and the correlation between the percentage of apoptosis and disease evolution (ex vivo percentage of
CD4 T cells) is shown. (E ) FACS dot plots representative of the analysis of TNF-RI-induced
apoptosis in PBMC from a control donor and an HIV-infected patient. Apoptosis in CD4 T cells is
measured by incorporation of 7-AAD into the nucleus. TNF-RI ligation induces apoptotic cell death
in T cells from the patient although it has no e¨ect in those from the control donor.
