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Figure 5.2. B/T cocultures increase the percentage of gp120
CD4
T cells. (A)10
4
HIV-1 in-
fected T cells were cultured alone or with 10
5
phorbol dibutyrate-activated B cells for 4 days. On
day 4, cell populations were analyzed for cell surface gp120 expression. Two-color FACScan anal-
ysis shows staining with IgG1-biotin and CD4-FITC (¯uorescein isothiocyanate) (upper plots) and
110.H-biotin and CD4-FITC (lower plots). (B) Expression of gp120 (bold line) and IgG1 (dotted
line) in gated CD4
T cells (R2 or R5 gates) is shown. Representative of two independent experi-
ments.
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