Biology Reference
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F i g u r e 5.1. Detection of in vitro HIV-infected B cells. (A±D) In situ hybridization to detect HIV
replicating in B cells. B cells were stimulated by phorbol ester for 2 days and infected, then cultured
in the presence of IL-2 plus IL-4. (A) Chronically infected T-cell line H9V3. (B) Negative control,
infected B cells on day 2 postinfection were hybridized with the positive strand probe. (C ) B cells
showing HIV replication on day 2 postinfection. (D) Isolated HIV
cells on day 2 postinfection.
Original magni®cation X100. Representative of three independent experiments. (E ) PCR detection
of HIV provirus. B cells were stimulated by phorbol ester for 2 days and infected and then cultured
in the presence of CD40 mAb and IL-4. One microgram of DNA was subjected to PCR. 1, Unin-
