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UPGMA
AireCand117
0.05 changes
PMos115
Estoril43
Roca7
Roca1112
AireCand113
SCruz106
SRitaLou104
Estoril44
Abano49
Montej3530
Abano51
Montej3529
Sobral110
Montej3526
SRitaLou103
Roca2301w
Roca2301p
SReiCoimbr108
Avecasta87
Montej3516
PAmarela48
Montej3528
Montej3527
AireCand114
Montej3531
A
B
Fig. 2. A : Unrooted dendogram of UPGMA for 26 individuals of Silene longicilia ssp.
cintrana and S. longicilia ssp. longicilia (Nei & Li distance). Legends refer to the
geographic origin of the material, cf. [3] for details. B : Principal coordinates analysis of
twenty six individual genotypes (AFLPs) of Silene longicilia ssp. longicilia and S.
longicilia ssp. cintrana , for eigenvalues and percentage of variance retained by axis see
[3].
For all the topics mentioned a bioinformatics approach was used either by aligning and
editing sequences with Sequence Navigator and Autoassembler (PE Applied Biosystems,
Inc.) or performing parsimony analysis (PAUP 4.0 ȕ for Macintosh, Swofford, 1998).
Genetic fingerprints comparison and analysis was done using Genescan and Genotyper
software (PE Applied Biosystems Inc.). Also other bioinformatics methods were applied
like Neighbour Joining (PAUP 4.0 ȕ) and multivariate methods (UPGMA and PCoA).
3. Results and Discussion
Global relationships within the section analysed with AFLPs are depicted in Fig.1.
Molecular and morphological methods demonstrated that Silene section Siphonomorpha is
a group of closely related species, with S. nutans being the most distinct taxon [3]. Thus,
these species were considered better treated as two instead of one section: section Italicae
and section Siphonomorpha , the former including thirteen of the species analysed and the
latter including two species, S. nutans and S. viridiflora .
The first section corresponds to a group of entities probably not behaving as biological
species, but including several endemics contributing to global biodiversity. The low level of
divergence within section Italicae and frequently common plastid markers are evidence of a
recent shared common origin.
Study of S. longicilia ssp. cintrana and S. rothmaleri revealed differences in
trichome length and density and hilum cell morphology. In both species the 18S-5.8S-26S
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